Some biochemists have claimed that tryptic peptides

Some biochemists have claimed that tryptic peptides that are derived from the interior of globular proteins elute later from reversed-phase C-18 columns than do tryptic peptides derived from the exterior of proteins. Provide a justification for this claim.     https://www.studyhelpus.com/Questions & Answers/tryptic-peptides/

Consider the role of sodium dodecylsulfate

Consider the role of sodium dodecylsulfate, CH₃(CH₂)₁₁OSO₃^- Na⁺, in SDS-PAGE. Suppose a biochemist were to explore the use of alternative detergents in PAGE experiments to obtain better molecular weight estimates of proteins. As alternatives to SDS, she decided to try the following: Dodecanol, which is CH₃(CH₂)₁₁ Considering the molecular mechanism of SDS action in molecular weight determination, discuss how dodecanol would behave as a substitute for SDS. Would it be expected to lead to reliable molecular weight estimates? Explain.     https://www.studyhelpus.com/Questions & Answers/sodium-dodecylsulfate/

Docecylamine hydrochloride

Docecylamine hydrochloride, which is CH₃(CH₂)₁₁NH₃⁺ Cl^-. Compare and contrast this compound with SDS in how it would affect the proteins and the electrophoretic process. Should you expect it to give reliable molecular weight estimates? Explain.     https://www.studyhelpus.com/Questions & Answers/docecylamine-hydrochloride/

The Anfinsen experiment with Ribonuclease A

The Anfinsen experiment with Ribonuclease A established the general principle that denatured protein can spontaneously refold under appropriate conditions. In one experiment, RNAase A was simultaneously reduced with b-mercaptoethanol and denatured with urea. Residual bME was dialyzed away, and the protein was oxidized in air. Following oxidation, the urea was dialyzed away. The resulting protein was enzymatically inactive. Explain the lack of activity.     https://www.studyhelpus.com/Questions & Answers/ribonuclease-a/

Consider the role of Bis-acrylamide in PAGE

Consider the role of Bis-acrylamide in PAGE. The recipe for an acrylamide gel typically uses 1 mole of Bis-acrylamide for every 20 moles of acrylamide. Suppose you were to double the amount of Bis-acrylamide in the recipe (a 2:20 ratio instead of a 1:20 ratio). Predict the effect of this change on the migration rates of the proteins through the gel. Justify your prediction.   https://www.studyhelpus.com/Questions & Answers/bis-acrylamide/

SDS gel electrophoresis showed that this inactive form

SDS gel electrophoresis showed that this inactive form of the enzyme contained species with sizes that corresponded to dimers and trimers of RNAase A. Explain why this is not surprising.     https://www.studyhelpus.com/Questions & Answers/sds-gel/

When a “trace” of bME was added to this inactive enzyme

When a “trace” of bME was added to this inactive enzyme, activity slowly appeared, until activity was fully restored. Explain why the restoration of activity occurred.     https://www.studyhelpus.com/Questions & Answers/inactive-enzyme/