Consider the role of sodium dodecylsulfate
Consider the role of sodium dodecylsulfate, CH3(CH2)11OSO3- Na+, in SDS-PAGE. Suppose a biochemist were to explore the use of alternative detergents in PAGE experiments to obtain better molecular weight estimates of proteins. As alternatives to SDS, she decided to try the following:
Dodecanol, which is CH3(CH2)11 Considering the molecular mechanism of SDS action in molecular weight determination, discuss how dodecanol would behave as a substitute for SDS. Would it be expected to lead to reliable molecular weight estimates? Explain.
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