Consider the role of Bis-acrylamide in PAGE
Consider the role of Bis-acrylamide in PAGE. The recipe for an acrylamide gel typically uses 1 mole of Bis-acrylamide for every 20 moles of acrylamide. Suppose you were to double the amount of Bis-acrylamide in the recipe (a 2:20 ratio instead of a 1:20 ratio). Predict the effect of this change on the migration rates of the proteins through the gel. Justify your prediction.
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